ExomeSEQ: bringing reads on-target
March 11th, 2010 § Leave a Comment
It is surprising to look at some of the numbers from exome capture followed by sequencing projects. The nature paper on exome-seq to identify cause of mendelian disorder has as low as 47% of uniquely mapping reads actually mapping on-target. Another study of 12 exome sequences also has a capture specificity of as low as 31%.
Why are these numbers so low?
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